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Normalization to reference samples

 

To normalize the expressions in samples to the expression in a reference sample, select Normalize with reference sample(-s) in the Pre-processing menu in the Data editor to open a dialog box. Select the sample to normalize in the left panel, select the appropriate reference sample(s) in the right panel, and press Select. Repeat this for all samples that should be normalized so that all samples that should be normalized have type Norm. (normalized) in the left panel, and all reference samples type Ref. (reference). It is possible to select multiple samples in either panel (hold down Ctrl or Shift while selecting), and you can select different reference samples for different target samples. When you have defined all target and reference sample relationships, press Apply to execute normalization.

 

    

 

GenEx will color the normalized columns (genes) yellow and, if the Remove reference sample check box in the dialog box was ticked, remove the reference sample from the sheet.