Contents - Index

Introduction

Congratulations! You have purchased the most powerful software for real-time quantitative PCR (qPCR) expression profiling. GenEx has all the features you need for routine qPCR expression profiling and much more:

• Excel compatible Data editor
• Normalization to sample amounts
• Geometric normalization with reference genes
• Conversion to fold changes
• Mean-centering of data
• Autoscaling of data
• Control panel 
• Data manager 
• 2D plots
• 3D plots
• Bar plot
• Box plot
• geNorm to find reference genes
• Normfinder to find reference genes  
• Scatter plot 
• Principal Component Analysis (**)
• Potential Curves (*)
• Hierarchical clustering 
• Heatmap analysis (**)
• Kohonen Self Organizing Map (**)
• Artificial Neural Networks (ANN) (*)
• Support Vector Machine (SVM) (*)
• Standard Curve 
• Reverse Calibration (**)
• Level of Detection (LOD) (**)
• Partial least square (*)
• 3-way decomposition (*)
• Descriptive statistics
• Parametric and non-parametric t-test
• One-way ANOVA 
• Two-way ANOVA (**)
• Nested ANOVA (**)
• Spearman and Pearson correlation 
• Choose sample size (**)  
• Choose optimal experimental design (*)

(*) Only in GenEx Enterprise

(**) Only in GenEx Pro/Enterprise

There are several tutorials available online. For tutorials, more information about GenEx, latest updates, references, and hints, see the MultiD Analyses AB (MultiD) homepage: www.multid.se